Sequence: AGYLLGKLLOOLAAAALOOLL
| Experiment Id | EXP001382 |
|---|---|
| Paper | Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetr |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 25–50 nM siRNA |
| Mixing Ratio | MR 20:1 (CPP:siRNA) |
| Formulation Format | noncovalent CPP–RNA complex with chitosan-modified Fe3O4 magnetic nanoparticles (CTS@MNPs) |
| Formulation Components | CTS@Fe3O4 magnetic nanoparticles (chitosan-modified iron oxide MNPs) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U-87 MG-luc2 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown (luc2) |
| Output Value | slight improvement vs PF14–siRNA (reported) |
| Output Units | |
| Output Notes | PF14–siRNA–CTS@MNPs showed small improvement over PF14–siRNA in luc2 knockdown (in vitro). |
| Toxicity Notes | WST-1 assay: complexes reported biocompatible / no observed cytotoxicity in HeLa cells. |
| Curation Notes |