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EXP001383

Paper

Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetrating peptide (2018)

Peptide

PF221

Sequence: FLKLLKKFLFLKLLKKFL

RNA

siRNA

All experiment fields

Experiment Id EXP001383
Paper Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetr
Peptide PF221
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 25–50 nM siRNA (used in complex preparation)
Mixing Ratio MR 20:1 (CPP:siRNA)
Formulation Format noncovalent CPP–RNA complex with chitosan-modified Fe3O4 magnetic nanoparticles (CTS@MNPs)
Formulation Components CTS@Fe3O4 magnetic nanoparticles (chitosan-modified iron oxide MNPs)
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Not reported (physicochemical characterization only)
Animal Model
Administration Route
Output Type physicochemical characterization (TEM/DLS/zeta)
Output Value
Output Units
Output Notes PF221–siRNA–CTS@MNPs prepared and characterized (TEM/DLS/zeta); no in vitro functional knockdown assay reported for PF221–siRNA in this paper.
Toxicity Notes WST-1 assay: complexes reported biocompatible / no observed cytotoxicity in HeLa cells.
Curation Notes