Vitamin B12 as a carrier of peptide nucleic acid (PNA) into bacterial cells (2017)
Sequence: KFFKFFKFFK
PNA (antisense oligonucleotide)
| Experiment Id | EXP001384 |
|---|---|
| Paper | Vitamin B12 as a carrier of peptide nucleic acid (PNA) into bacterial cells |
| Peptide | (KFF)3K |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0–16 µM (tested range; overnight treatment) |
| Rna Concentration | 0–16 µM (as conjugate) |
| Mixing Ratio | Covalent 1:1 (carrier:PNA) |
| Formulation Format | Covalent carrier–PNA conjugate |
| Formulation Components | (KFF)3K–PNA conjugate (anti-mrfp1) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | E. coli K-12 MG1655 (pBBR(rfp)) |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter inhibition (normalized RFP fluorescence, RFU) |
| Output Value | 0.29 |
| Output Units | |
| Output Notes | RFU shown is mean approx. at 16 µM after overnight incubation; lower RFU indicates stronger antisense inhibition of RFP expression. |
| Toxicity Notes | No bacterial growth inhibition reported (OD600 unchanged) at concentrations used for delivery. |
| Curation Notes |