Sequence: KHKHKHKHKHKHKHKHKHKKLFKKILKYL
| Experiment Id | EXP001400 |
|---|---|
| Paper | Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nucle |
| Peptide | KH9-BP100 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1.05 µM KH9-BP100 (N/P=2.0) |
| Rna Concentration | 217.6 nM siGFPS1 (N/P=2.0) |
| Mixing Ratio | N/P=2.0 |
| Formulation Format | Electrostatic siRNA/CPP complex (polyplex), prepared then diluted in spray solution |
| Formulation Components | Spray solution: 5% (w/v) sucrose + 0.05% (v/v) Silwet L-77 |
| Size Nm | 263.90 |
| Zeta Mv | -27.10 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Transgenic tomato (MicroTom) leaf cells overexpressing GFP (foliar spraying; 3 days after spraying) |
| Administration Route | Foliar spray (leaf surface spraying) |
| Output Type | GFP fluorescence; GFP protein abundance; GFP transcript levels |
| Output Value | GFP fluorescence decreased ~83% at 3 DAS; GFP protein 28% remained (immunoblot); GFP transcripts decreased by 59% at 3 DAS |
| Output Units | |
| Output Notes | Functional gene silencing in leaf cells via sprayed siRNA/CPP complexes. |
| Toxicity Notes | |
| Curation Notes |