Sequence: KH9-OEP34: KHKHKHKHKHKHKHKHKHMFAFQYLLVM | BP100: KKLFKKILKYL
| Experiment Id | EXP001402 |
|---|---|
| Paper | Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nucle |
| Peptide | KH9-OEP34 (CTP) + BP100 (CPP) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | KH9-OEP34 1.32 µM; BP100 4.68 µM (N/P=2.5) |
| Rna Concentration | 217.6 nM siGFPS1 (N/P=2.5) |
| Mixing Ratio | N/P=2.5 (siGFPS1/KH9-OEP34/BP100) |
| Formulation Format | Clustered siRNA/CTP/CPP electrostatic complex, prepared then diluted in spray solution |
| Formulation Components | Spray solution: 5% (w/v) sucrose + 0.05% (v/v) Silwet L-77 |
| Size Nm | 112.70 |
| Zeta Mv | 25.50 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Transplastomic Nicotiana tabacum line overexpressing eGFP in chloroplasts (foliar spraying; 3 days after spraying) |
| Administration Route | Foliar spray (leaf surface spraying) |
| Output Type | eGFP/chlorophyll fluorescence in chloroplasts; GFP protein (immunoblot); eGFP transcript levels |
| Output Value | Reduced eGFP fluorescence/protein/transcripts in chloroplasts (N/P-ratio dependent; see Fig 6c–g) |
| Output Units | |
| Output Notes | Chloroplast-targeted gene silencing using siRNA/CTP/CPP complexes at N/P=2.5. |
| Toxicity Notes | |
| Curation Notes |