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EXP001423

Paper

Cell-penetrating peptide-siRNA conjugate loaded YSA-modified nanobubbles for ultrasound triggered siRNA delivery (2015)

Peptide

Penetratin-derived CPP

Sequence: CKRRMKWKK

RNA

siRNA

All experiment fields

Experiment Id EXP001423
Paper Cell-penetrating peptide-siRNA conjugate loaded YSA-modified nanobubbles for ultrasound triggered si
Peptide Penetratin-derived CPP
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 100 nM final c-Myc siRNA (as CPP–siRNA conjugate)
Mixing Ratio Covalent 1:1 CPP:siRNA via disulfide bond
Formulation Format Free CPP–siRNA conjugate
Formulation Components CPP–siRNA conjugate (disulfide-linked; reducible by cytosolic GSH) (no nanobubble; no targeting ligand)
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells MCF-7 (human breast adenocarcinoma; EphA2-positive)
Animal Model
Administration Route
Output Type In vitro functional effect: c-Myc mRNA/protein and apoptosis
Output Value
Output Units
Output Notes CPP–siRNA induced c-Myc knockdown and apoptosis in vitro, but was less effective than CPP–siRNA/YSA-NB (+US).
Toxicity Notes
Curation Notes