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EXP001427

Paper

Cell-penetrating peptide-siRNA conjugate loaded YSA-modified nanobubbles for ultrasound triggered siRNA delivery (2015)

Peptide

Penetratin-derived CPP

Sequence: CKRRMKWKK

RNA

siRNA

All experiment fields

Experiment Id EXP001427
Paper Cell-penetrating peptide-siRNA conjugate loaded YSA-modified nanobubbles for ultrasound triggered si
Peptide Penetratin-derived CPP
Delivery Success Class no
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 10 mg/kg c-Myc siRNA (as CPP–siRNA conjugate) via tail vein
Mixing Ratio Covalent 1:1 CPP:siRNA via disulfide bond
Formulation Format Free CPP–siRNA conjugate
Formulation Components CPP–siRNA conjugate (disulfide-linked; reducible by cytosolic GSH) (no nanobubble; no targeting; no ultrasound)
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model BALB/c nude mice with MCF-7 xenograft tumors
Administration Route Intravenous (tail vein)
Output Type Tumor growth (relative tumor volume) and tumor c-Myc expression
Output Value
Output Units
Output Notes Only modest tumor inhibition reported compared with CPP–siRNA/YSA-NB (+US); attributed to instability/non-specific distribution.
Toxicity Notes No major acute toxicity reported in body weight monitoring.
Curation Notes