Sequence: RRRRRRRRR
| Experiment Id | EXP001447 |
|---|---|
| Paper | Super-resolution Imaging of Structure, Molecular Composition, and Stability of Single Oligonucleotid |
| Peptide | L-nona-arginine (R9) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~48–50 µM peptide (with 0.045 µM mRNA) for dSTORM prep; uptake exp used 25 µM peptide (with 45 pM mRNA) for cell uptake assays |
| Rna Concentration | dSTORM prep: 0.045 µM mRNA; uptake exp: 45 pM mRNA (10× lower than dSTORM) |
| Mixing Ratio | N/P (charge) ratio = 5. Polyplexes formed by mixing equal volumes of diluted peptide and mRNA in water (simultaneous expulsion mixing). |
| Formulation Format | CPP/mRNA polyplexes (noncovalent electrostatic complexes) for imaging and uptake assays |
| Formulation Components | R9 peptide + luciferase mRNA (Cy5-labeled for imaging). For dSTORM: AlexaFluor488-R9 mixed with unlabeled R9 (1.25% labeled). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells (DSMZ ACC-57) for uptake imaging; polyplex characterization on glass coverslips (dSTORM) and DLS in water |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake/internalization (confocal microscopy, 1 h) + structural characterization (two-color dSTORM; polyplex size/composition) + serum stability (for N/P 5) |
| Output Value | |
| Output Units | |
| Output Notes | At N/P 5, polyplexes showed higher R9 incorporation and maximal uptake; additional peptide above saturation remained free. Serum stability tested primarily at N/P 5 (rapid peptide loss at 1 min; payload loss by 1 h in 20% FBS). Polyplex radii typically ~60–80 nm by dSTORM (NP1 more polydisperse with population >100 nm). |
| Toxicity Notes | Polyplex peptide concentrations used for uptake were reported non-cytotoxic across N/P ratios (viability assay in SI). |
| Curation Notes |