CTX-R10 (chlorotoxin + deca-arginine)
Sequence: CTX (not specified) ; RRRRRRRRRR
| Experiment Id | EXP001449 |
|---|---|
| Paper | Iron oxide nanoparticles decorated with functional peptides for a targeted siRNA delivery to glioma |
| Peptide | CTX-R10 (chlorotoxin + deca-arginine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | NP-CTX-R10 used at 20 µg/mL (Fe) for uptake assays; NP amounts varied for complexation tests |
| Rna Concentration | siRNA feed concentration 5–50 µg/mL (complexation/size tests); functional assay used siRFP (amount not specified in SI) |
| Mixing Ratio | NP:siRNA mass ratio 20:1 to 0.5:1 (HEPES pH 7.4); complexes incubated in serum media (37°C, 4 h) for stability test |
| Formulation Format | Peptide-decorated iron oxide nanoparticle/siRNA complexes (NP-CTX-R10/siRNA) |
| Formulation Components | Iron oxide NP + CCP-PEI polymer coating + CTX targeting peptide + R10 CPP; siRNA electrostatically complexed (NP:siRNA mass ratio varied). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | C6 rat glioma cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional effect: reporter knockdown (RFP intensity by flow cytometry after siRFP delivery) + uptake (Cy5 flow cytometry) |
| Output Value | |
| Output Units | |
| Output Notes | Supplementary Fig. S3 shows reduced RFP fluorescence in C6 cells treated with NP-CTX-R10 + siRFP compared with RFP-only control; uptake quantified by flow cytometry (Supplementary Methods). |
| Toxicity Notes | |
| Curation Notes |