Chaperonin–Dendrimer Conjugates for siRNA Delivery (2016)
Sequence: GRKKRRQRRRPPQ-NH2
| Experiment Id | EXP001452 |
|---|---|
| Paper | Chaperonin–Dendrimer Conjugates for siRNA Delivery |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | Transfection: final 100 nM siRNA (10 pmol per well in 100 µL). |
| Mixing Ratio | TAT–THS–PAMAM loaded with KIF11 siRNA or scrambled siRNA; delivered using reversed siRNA transfection protocol. |
| Formulation Format | CPP-decorated protein cage–dendrimer/siRNA complexes (TAT–THS–PAMAM–siRNA) |
| Formulation Components | THS protein cage + encapsulated PAMAM G4 + surface-conjugated TAT peptide + siRNA (KIF11 or scrambled control). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | PC-3 prostate cancer cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional effect: KIF11 siRNA delivery reduced cell viability vs scrambled control (72 h). Uptake shown by flow cytometry and confocal imaging of TAT–THS–Atto647. |
| Output Value | |
| Output Units | |
| Output Notes | TAT decoration increased PC-3 uptake (~630× median fluorescence vs background by flow cytometry). Confocal imaging shows internalization at 2 h and low co-localization with LysoTracker (escape ambiguous). KIF11 siRNA-loaded TAT–THS–PAMAM reduced viability to ~69% of untreated and ~79% vs scrambled control. |
| Toxicity Notes | |
| Curation Notes |