Human DMBT1-Derived Cell-Penetrating Peptides for Intracellular siRNA Delivery (2017)
Sequence: GRVEVLYRGSW
| Experiment Id | EXP001453 |
|---|---|
| Paper | Human DMBT1-Derived Cell-Penetrating Peptides for Intracellular siRNA Delivery |
| Peptide | SRCRP2-11 (DMBT1-derived CPP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Final peptide 6.0 µM (with 100 nM siRNA) for knockdown assay; molar ratio 60:1 (peptide:siRNA). |
| Rna Concentration | Final siRNA 100 nM in cell culture (reverse transfection, 96 h). |
| Mixing Ratio | Peptide/siRNA molar ratio 60:1 (peptide:siRNA). Complexes formed in PBS (30 min, 37°C) before use. |
| Formulation Format | Self-assembled peptide/siRNA nanocomplexes (noncovalent electrostatic complexes) |
| Formulation Components | SRCRP2-11 (DMBT1-derived CPP) peptide + tdTomato1 siRNA (or non-targeting control); complexes delivered in (reported) FBS-free conditions for knockdown readout. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF7 tdTomato stable recombinant cells (knockdown); MCF7 cells for uptake (FITC-peptide + Cy3-siRNA confocal, 24 h). |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional effect: tdTomato fluorescence knockdown at 96 h (normalized to viability) + uptake confirmed by confocal microscopy and fluorescence measurements (24 h) for peptide/Cy3-siRNA complexes. |
| Output Value | ~48% tdTomato knockdown at optimal ratio. |
| Output Units | |
| Output Notes | Optimal knockdown at 60:1. Reported ~48% tdTomato knockdown vs untreated at 96 h; non-targeting siRNA complexes did not significantly change fluorescence. |
| Toxicity Notes | No clear morphological toxicity reported in confocal images; viability normalization performed for knockdown readout. |
| Curation Notes |