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EXP001482

Paper

Interference of hepatitis C virus replication in cell culture by antisense peptide nucleic acids targeting the X-RNA (2011)

Peptide

Tat48-60

Sequence: GRKKRRQRRRPPQ

RNA

antisense PNA

All experiment fields

Experiment Id EXP001482
Paper Interference of hepatitis C virus replication in cell culture by antisense peptide nucleic acids tar
Peptide Tat48-60
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration (not explicitly stated; treated as in Fig. 2b protocol)
Rna Concentration
Mixing Ratio
Formulation Format covalent CPP–PNA conjugate
Formulation Components HIV-1 Tat48–60 peptide + antisense PNA (SL3, 13-mer) (covalent)
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells R-1 (Huh7-derived HCV subgenomic replicon, genotype 1b)
Animal Model
Administration Route
Output Type HCV genome copy number (qRT-PCR)
Output Value Suppression similar to Tat-PNA-SL1
Output Units
Output Notes Tat-PNA-SL3 suppressed HCV RNA replication in R-1 cells at a level similar to Tat-PNA-SL1.
Toxicity Notes
Curation Notes