Sequence: GRKKRRQRRRPPQ
| Experiment Id | EXP001486 |
|---|---|
| Paper | Interference of hepatitis C virus replication in cell culture by antisense peptide nucleic acids tar |
| Peptide | Tat48-60 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10–20 µM |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | covalent CPP–PNA conjugate |
| Formulation Components | HIV-1 Tat48–60 peptide + antisense PNA (SL3-15, 15-mer) (covalent) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Huh7 infected with HCV JFH1 (genotype 2a), MOI 0.3 FFU/cell |
| Animal Model | |
| Administration Route | |
| Output Type | HCV genome copy number (qRT-PCR) |
| Output Value | Dose-dependent suppression; 20 µM comparable to IFN-α (100 U/mL) |
| Output Units | |
| Output Notes | In HCV-infected Huh7 cells, Tat-PNA-SL3-15 suppressed HCV replication dose-dependently; no significant cytotoxicity up to 20 µM (<10% at 20 µM). |
| Toxicity Notes | <10% cytotoxicity at 20 µM (MTS assay) |
| Curation Notes |