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EXP001487

Paper

Interference of hepatitis C virus replication in cell culture by antisense peptide nucleic acids targeting the X-RNA (2011)

Peptide

Tat48-60

Sequence: GRKKRRQRRRPPQ

RNA

antisense PNA (control)

All experiment fields

Experiment Id EXP001487
Paper Interference of hepatitis C virus replication in cell culture by antisense peptide nucleic acids tar
Peptide Tat48-60
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration 10–20 µM (matched to Tat-PNA-SL3-15 conditions)
Rna Concentration
Mixing Ratio
Formulation Format covalent CPP–PNA conjugate
Formulation Components HIV-1 Tat48–60 peptide + control PNA (J5) (covalent)
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Huh7 infected with HCV JFH1 (genotype 2a), MOI 0.3 FFU/cell
Animal Model
Administration Route
Output Type HCV genome copy number (qRT-PCR)
Output Value No significant antiviral effect
Output Units
Output Notes Tat-PNA-J5 control PNA showed no significant antiviral effect in HCV-infected Huh7 cells.
Toxicity Notes
Curation Notes