Sequence: Ac-RQIKIWFQNRRMKWKKKC
| Experiment Id | EXP001492 |
|---|---|
| Paper | Comparing Agent-Based Delivery of DNA and PNA Forced Intercalation (FIT) Probes for Multicolor mRNA |
| Peptide | Penetratin |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 2 µM (as Pen_FIT-DNA1 conjugate; 30 min incubation) |
| Rna Concentration | 2 µM (DNA probe; in Pen_FIT-DNA1 conjugate) |
| Mixing Ratio | 1:1 (covalent conjugate) |
| Formulation Format | covalent CPP–DNA FIT probe conjugate (disulfide linker) |
| Formulation Components | Pen_FIT-DNA1 (penetratin–FIT-DNA1) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Flp-In 293 T-REx (HEK293) reporter cells with inducible F-tagged mCherry mRNA (+/- doxycycline) |
| Animal Model | |
| Administration Route | |
| Output Type | Fluorescence microscopy mRNA imaging (TO emission); compare +dox vs -dox |
| Output Value | Weak fluorescence; remained low with or without mRNA induction (+dox/-dox). |
| Output Units | |
| Output Notes | DNA–penetratin conjugate did not provide clear target-dependent signal; background remained low but signal weak. |
| Toxicity Notes | |
| Curation Notes |