Sequence: Ac-KETWFETWFTEWSQPKKKRKVC
| Experiment Id | EXP001497 |
|---|---|
| Paper | Comparing Agent-Based Delivery of DNA and PNA Forced Intercalation (FIT) Probes for Multicolor mRNA |
| Peptide | Pep2 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1 µM (as Pep2_FIT-PNA1 conjugate; 30 min incubation) |
| Rna Concentration | 1 µM (PNA probe; in Pep2_FIT-PNA1 conjugate) |
| Mixing Ratio | 1:1 (covalent conjugate) |
| Formulation Format | covalent CPP–PNA FIT probe conjugate (disulfide linker) |
| Formulation Components | Pep2_FIT-PNA1 (Pep2–FIT-PNA1) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Flp-In 293 T-REx (HEK293) reporter cells with inducible F-tagged mCherry mRNA (+/- doxycycline) |
| Animal Model | |
| Administration Route | |
| Output Type | Fluorescence microscopy mRNA imaging (TO emission); compare +dox vs -dox |
| Output Value | Spot-like fluorescence in induced and non-induced cells; artefacts prevented reliable RNA imaging. |
| Output Units | |
| Output Notes | CPP–PNA conjugate produced strong background spots/aggregates similar to other CPP conjugates. |
| Toxicity Notes | |
| Curation Notes |