Sequence: RRRRRRRR
| Experiment Id | EXP001499 |
|---|---|
| Paper | Comparing Agent-Based Delivery of DNA and PNA Forced Intercalation (FIT) Probes for Multicolor mRNA |
| Peptide | Octaarginine (R8) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | Complexation; complete DNA binding reported at charge ratio P/N=16 (R8:DNA). |
| Rna Concentration | 2 µM (DNA probe during imaging experiments; complexed with R8) |
| Mixing Ratio | P/N=16 (positive/negative charge ratio; complexation condition) |
| Formulation Format | noncovalent electrostatic complex (R8 + DNA FIT probe) |
| Formulation Components | R8 + FIT-DNA1 complex |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Flp-In 293 T-REx (HEK293) reporter cells with inducible F-tagged mCherry mRNA (+/- doxycycline) |
| Animal Model | |
| Administration Route | |
| Output Type | Fluorescence microscopy mRNA imaging (TO emission); compare +dox vs -dox |
| Output Value | Large fluorescent spots (artefacts); RNA imaging impossible. |
| Output Units | |
| Output Notes | Octaarginine complexation led to spot-like aggregates/background in both induced and non-induced cells. |
| Toxicity Notes | |
| Curation Notes |