LMWP (low molecular weight protamine)
Sequence: VSRRRRRRGGRRRR
| Experiment Id | EXP001507 |
|---|---|
| Paper | Intracellular delivery of messenger RNA by recombinant PP7 virus-like particles carrying low molecul |
| Peptide | LMWP (low molecular weight protamine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | VLPs 100 nM/mL (for GFP expression assay); 10 µg/mL for FITC-uptake assay |
| Rna Concentration | N/A (mRNA encapsidated inside VLP; not separately dosed) |
| Mixing Ratio | N/A (genetic packaging/display) |
| Formulation Format | virus-like particle (PP7 VLP) displaying CPP and encapsidating mRNA |
| Formulation Components | PP7 VLP (2PP7) displaying LMWP on surface + packaged GFP mRNA (2PP7-Protamine-GFP) |
| Size Nm | 30.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | RM-1 mouse prostate cancer cells (uptake and GFP expression); Cos-7 cells (cytotoxicity) |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (GFP fluorescence microscopy/confocal) + GFP mRNA (qPCR) + uptake (FITC imaging) |
| Output Value | GFP fluorescence in most RM-1 cells at 24 h; GFP mRNA detected at 24/48 h; FITC-labeled VLP uptake observed at 12 h |
| Output Units | |
| Output Notes | LMWP-displaying PP7 VLPs penetrated RM-1 cells (FITC imaging) and delivered packaged GFP mRNA that was translated into GFP protein (fluorescence/confocal) with GFP mRNA detected by qPCR. |
| Toxicity Notes | No dose-dependent cytotoxicity in Cos-7 cells; viability >95% even at 800 µg/mL VLPs (24–48 h, CCK-8). |
| Curation Notes |