Back to browse

EXP001509

Paper

Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complexes detected by fluorescence correlation spectroscopy (2018)

Peptide

PF14K-CR6G

Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLLK(εNH-CR6G)

RNA

siRNA

All experiment fields

Experiment Id EXP001509
Paper Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complex
Peptide PF14K-CR6G
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence medium
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration 100 nM (calculated from MR10 & 10 nM siRNA)
Rna Concentration 10 nM
Mixing Ratio MR10 (peptide:siRNA)
Formulation Format non-covalent peptide/siRNA complex
Formulation Components PF14K-CR6G + anti-Luc siRNA
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells U87-Luc glioblastoma cells
Animal Model
Administration Route
Output Type gene knockdown (luciferase assay)
Output Value
Output Units
Output Notes Labeled peptide showed no significant difference vs PF14 in anti-Luc siRNA delivery (Fig. S10).
Toxicity Notes
Curation Notes