Sequence: PF14 + PF14K-CR6G (see individual sequences)
| Experiment Id | EXP001510 |
|---|---|
| Paper | Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complex |
| Peptide | PF14 + PF14K-CR6G (tracer) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 250 nM complex (PF14/PF14K-CR6G/Cy5-siRNA, 10:1:1) |
| Rna Concentration | |
| Mixing Ratio | 10:1:1 (PF14:PF14K-CR6G:Cy5-siRNA) |
| Formulation Format | non-covalent peptide/siRNA complex (dual-labeled for FCCS) |
| Formulation Components | unlabeled PF14 + PF14K-CR6G + Cy5-siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | PC12 cells |
| Animal Model | |
| Administration Route | |
| Output Type | uptake/colocalization at plasma membrane (confocal; FCCS cross-correlation) |
| Output Value | Co-localization observed; positive cross-correlation increases over time |
| Output Units | |
| Output Notes | Complex presence at plasma membrane confirmed by positive cross-correlation; confocal shows red/green co-localization (orange). |
| Toxicity Notes | |
| Curation Notes |