Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
plasmid DNA (DNA/RNA layered vector)
| Experiment Id | EXP001513 |
|---|---|
| Paper | Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penet |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~52.5 µM (charge ratio DNA:PF6 = 1:5) |
| Rna Concentration | 1 µg per well (24-well; ~2 cm^2 growth area) |
| Mixing Ratio | charge ratio 1:5 (DNA:PF6) |
| Formulation Format | electrostatic CPP/DNA nanoparticles |
| Formulation Components | PF6 + SFV layered vector plasmid DNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | BHK-21 (baby hamster kidney) |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase expression (Fluc activity, 8 h post-transfection) |
| Output Value | High Fluc activity; optimal at charge ratio 1:5 |
| Output Units | |
| Output Notes | PF6 delivered SFV DNA/RNA layered vectors; optimal DNA:PF6 charge ratio reported as 1:5 in BHK-21 cells. PF6-delivered DNA showed delayed nuclear entry vs Lipofectamine 2000 (~1.5 h). |
| Toxicity Notes | |
| Curation Notes |