Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
viral RNA (in vitro transcript)
| Experiment Id | EXP001514 |
|---|---|
| Paper | Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penet |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~52.5 µM (charge ratio RNA:PF6 = 1:5) |
| Rna Concentration | 1 µg per well (24-well) |
| Mixing Ratio | charge ratio 1:5 (RNA:PF6) |
| Formulation Format | electrostatic CPP/RNA nanoparticles |
| Formulation Components | PF6 + SFV RNA transcript |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Primary mouse embryonic fibroblasts (MEF) |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase expression (Fluc activity, 24 h post-transfection) |
| Output Value | Measurable Fluc; optimal at 1:5 in MEFs |
| Output Units | |
| Output Notes | In interferon-competent primary MEFs, PF6 enabled transfection with SFV RNA; 1:5 charge ratio used as optimal for both DNA and RNA. |
| Toxicity Notes | |
| Curation Notes |