Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
plasmid DNA (DNA/RNA layered vector)
| Experiment Id | EXP001515 |
|---|---|
| Paper | Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penet |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~52.5 µM (charge ratio DNA:PF6 = 1:5) |
| Rna Concentration | 1 µg per well (24-well) |
| Mixing Ratio | charge ratio 1:5 (DNA:PF6) |
| Formulation Format | electrostatic CPP/DNA nanoparticles |
| Formulation Components | PF6 + SFV layered vector plasmid DNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Primary mouse embryonic fibroblasts (MEF) |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase expression (Fluc activity, 24 h post-transfection) |
| Output Value | Measurable Fluc; PF6 comparable to Lipofectamine 2000 for DNA in MEFs |
| Output Units | |
| Output Notes | PF6 delivered SFV DNA/RNA layered vectors into primary MEFs with 1:5 charge ratio; reporter expression assessed at 24 h. |
| Toxicity Notes | |
| Curation Notes |