Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
replicon RNA (in vitro transcript)
| Experiment Id | EXP001516 |
|---|---|
| Paper | Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penet |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | (as optimized for RNA in BHK-21; 1:3 charge ratio) |
| Rna Concentration | 1 µg per well |
| Mixing Ratio | charge ratio 1:3 (RNA:PF6) |
| Formulation Format | electrostatic CPP/RNA nanoparticles |
| Formulation Components | PF6 + SFV replicon RNA (Fluc+EGFP) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | BHK-21 |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (EGFP-positive cells at 14 h; Fluc activity) |
| Output Value | ~7% EGFP-positive cells at 14 h (PF6) |
| Output Units | |
| Output Notes | Using SFV replicon RNA, PF6 transfection produced EGFP-positive cells (reported ~7% at 14 h). |
| Toxicity Notes | |
| Curation Notes |