Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
replicon plasmid DNA (DNA/RNA layered)
| Experiment Id | EXP001517 |
|---|---|
| Paper | Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penet |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | (as optimized for DNA in BHK-21; 1:5 charge ratio) |
| Rna Concentration | 1 µg per well |
| Mixing Ratio | charge ratio 1:5 (DNA:PF6) |
| Formulation Format | electrostatic CPP/DNA nanoparticles |
| Formulation Components | PF6 + CMV-layered SFV replicon plasmid (Fluc+EGFP) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | BHK-21 |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (EGFP-positive cells at 14 h; Fluc activity) |
| Output Value | ~17% EGFP-positive cells at 14 h (PF6) |
| Output Units | |
| Output Notes | PF6 delivered the DNA/RNA layered replicon plasmid with higher EGFP-positive fraction (~17% at 14 h) than replicon RNA under their conditions. |
| Toxicity Notes | |
| Curation Notes |