Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
| Experiment Id | EXP001518 |
|---|---|
| Paper | Transfection of Infectious RNA and DNA/RNA Layered Vectors of Semliki Forest Virus by the Cell-Penet |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | (charge ratio siRNA:PF6 = 1:40; exact µM not reported) |
| Rna Concentration | 100 nM siRNA in mixture (10.5 µL of 100 nM used per well per protocol) |
| Mixing Ratio | charge ratio 1:40 (siRNA:PF6) |
| Formulation Format | electrostatic CPP/siRNA nanoparticles |
| Formulation Components | PF6 + anti-EGFP siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-EGFP (stable EGFP) and CHO cells (pre-transfection before SFV infection) |
| Animal Model | |
| Administration Route | |
| Output Type | Functional knockdown + impact on subsequent SFV infection (confocal: nsP1 / nsP3-ZsGreen replication organelles) |
| Output Value | EGFP levels greatly diminished by 24 h (knockdown described); PF6 pre-transfection showed no adverse effect on subsequent SFV infection even at 2 h interval |
| Output Units | |
| Output Notes | Cells pre-transfected with PF6/anti-EGFP siRNA showed strong EGFP reduction by 24 h and did not show altered SFV replication organelle phenotype upon subsequent infection, unlike Lipofectamine 2000 at short intervals. |
| Toxicity Notes | |
| Curation Notes |