Sequence: LIRLWSHLIHIWFQNRRLKWKKK
| Experiment Id | EXP001524 |
|---|---|
| Paper | The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust, S |
| Peptide | EB1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Variable; example 2500 ng EB1 with 500 ng siRNA (5:1 w:w) for injections |
| Rna Concentration | Varied; 250–1000 ng per 10 µL for feeding; 500 ng per 4 µL for injection |
| Mixing Ratio | 5:1 w:w (EB1:siRNA); stability also tested at 1:1 and 2:1 w:w |
| Formulation Format | noncovalent EB1:siRNA complexes |
| Formulation Components | Incubated in MgES up to 2 h; analyzed by agarose gel |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | |
| Animal Model | Schistocerca gregaria midgut enzyme solution (MgES) ex vivo |
| Administration Route | |
| Output Type | siRNA stability in MgES (gel shift/degradation assay) |
| Output Value | EB1:siRNA complexes at 5:1 w:w remained stable up to 120 min in MgES |
| Output Units | |
| Output Notes | Complexes at 1:1 degraded rapidly; 2:1 partially stable; 5:1 stable for entire assay (Results 3.4; Figure 10). |
| Toxicity Notes | |
| Curation Notes |