Sequence: LIRLWSHLIHIWFQNRRLKWKKK
| Experiment Id | EXP001525 |
|---|---|
| Paper | The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust, S |
| Peptide | EB1 |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Adjusted to maintain 5:1 w:w; examples: 50–1500 ng EB1 per 10 µL |
| Rna Concentration | 10–300 ng dsRNA per 10 µL per day (5-day assay); also 8-day assay (single dose) |
| Mixing Ratio | 5:1 w:w (EB1:dsRNA) |
| Formulation Format | noncovalent EB1:dsRNA complexes |
| Formulation Components | PBS; force-feeding 10 µL/day |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Schistocerca gregaria (desert locust), adult |
| Administration Route | oral force-feeding (10 µL/day, 5 or 8 days) |
| Output Type | tub mRNA levels (qPCR) in midgut and brain |
| Output Value | No tub knockdown observed in midgut or brain at any tested dsRNA dose |
| Output Units | |
| Output Notes | EB1:dsRNA complexes failed to induce RNAi by feeding (Results 3.2; Figures 7–8). |
| Toxicity Notes | |
| Curation Notes |