Sequence: LIRLWSHLIHIWFQNRRLKWKKK
| Experiment Id | EXP001526 |
|---|---|
| Paper | The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust, S |
| Peptide | EB1 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 2500 ng EB1 per 4 µL injection (5:1 w:w with 500 ng siRNA) |
| Rna Concentration | 500 ng siRNA per 4 µL injection; boost injection at day 3 |
| Mixing Ratio | 5:1 w:w (EB1:siRNA) |
| Formulation Format | noncovalent EB1:siRNA complexes |
| Formulation Components | Sg-Ringer solution; 4 µL injection |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Schistocerca gregaria (desert locust), adult |
| Administration Route | intra-hemocoelic injection (body cavity); boost at day 3; dissect day 6 |
| Output Type | tub mRNA levels (qPCR) in midgut |
| Output Value | Significant tub transcript reduction vs EB1:dsLuc-siRNA control |
| Output Units | |
| Output Notes | Naked siRNA injection did not knock down tub, but EB1:siRNA complexes did (Results 3.5–3.6; Figures 11–12). |
| Toxicity Notes | |
| Curation Notes |