Sequence: LIRLWSHLIHIWFQNRRLKWKKK
| Experiment Id | EXP001527 |
|---|---|
| Paper | The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust, S |
| Peptide | EB1 |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1250 ng EB1 per 10 µL (with 250 ng siRNA) or 5000 ng EB1 per 10 µL (with 1000 ng siRNA) |
| Rna Concentration | 250 ng or 1000 ng siRNA per 10 µL per day (5-day assay) |
| Mixing Ratio | 5:1 w:w (EB1:siRNA) |
| Formulation Format | noncovalent EB1:siRNA complexes |
| Formulation Components | PBS; force-feeding 10 µL/day for 5 days |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Schistocerca gregaria (desert locust), adult |
| Administration Route | oral force-feeding (10 µL/day for 5 days) |
| Output Type | tub mRNA levels (qPCR) in midgut and brain |
| Output Value | No tub knockdown observed in midgut or brain at either siRNA dose |
| Output Units | |
| Output Notes | EB1:siRNA complexes failed to induce RNAi by feeding (Results 3.7; Figure 13). |
| Toxicity Notes | |
| Curation Notes |