Sequence: RQIKIWFQNRRMKWKK
| Experiment Id | EXP001540 |
|---|---|
| Paper | Intracellular delivery of antiviral shRNA using penetratin-based complexes effectively inhibits resp |
| Peptide | Penetratin (PEN) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | Not reported (penetratin:plasmid molar ratio 20:1) |
| Rna Concentration | Not reported |
| Mixing Ratio | molar ratio 20:1 (penetratin:plasmid) |
| Formulation Format | noncovalent CPP/DNA complexes (electrostatic) |
| Formulation Components | Penetratin + scrambled shRNA plasmid (pGFP-V-RS) |
| Size Nm | 164.00 |
| Zeta Mv | 8.70 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 infected with RSV A2 (MOI 0.1) |
| Animal Model | |
| Administration Route | |
| Output Type | Negative control for apoptosis/antiviral assays (PI staining; caspase 3/7) |
| Output Value | Caspase 3/7 activity remained high in infected penetratin-scramble group (similar to infected untreated) |
| Output Units | |
| Output Notes | Scrambled control used to show specificity of F-shRNA effect on apoptosis markers and viral outcomes. |
| Toxicity Notes | |
| Curation Notes |