Sequence: Ac-YKLXLKLXLKXLKLXLK-NH2
| Experiment Id | EXP001554 |
|---|---|
| Paper | Intracellular Delivery of Plasmid DNA Using Amphipathic Helical Cell-Penetrating Peptides Containing |
| Peptide | Pep4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 0.25 µg pDNA/well (96-well transfection assays); 1 µg pDNA/well (24-well uptake FACS); 0.5 µg pDNA/well (CLSM) |
| Mixing Ratio | N/P = 4 (molar ratio of peptide amino/guanidino groups to pDNA phosphates) |
| Formulation Format | electrostatic peptide/pDNA complexes (CPP nanoparticles) |
| Formulation Components | Pep4 + pDNA (luciferase reporter plasmid) |
| Size Nm | 209.00 |
| Zeta Mv | 33.90 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Huh-7 human hepatoma cells (primary assays; HeLa shown in Supplementary Fig. S1) |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (luciferase) ± uptake (Cy5-pDNA flow cytometry/CLSM) |
| Output Value | High transfection; increases with N/P; superior to jetPEI. |
| Output Units | |
| Output Notes | Pep2–Pep4 showed higher pDNA transfection efficiencies than other peptides and jetPEI; Pep2/Pep3 decreased with higher N/P, Pep4 increased with higher N/P. Time-course with gLuc pDNA showed continued increase/plateau through 72 h. |
| Toxicity Notes | Cytotoxicity ranking reported: Pep5 > Pep2, Pep3, Pep7 > Pep4, Pep6 > Pep1 (24 h viability assay). |
| Curation Notes |