Sequence: 4-branched dendrimer; arms ~RXRX (R-Ahx-R-Ahx-R); Lys branching; C-terminal Ahx-βAla-Cys
| Experiment Id | EXP001587 |
|---|---|
| Paper | Synthesis and Splice-Redirecting Activity of Branched, Arginine-Rich Peptide Dendrimer Conjugates of |
| Peptide | RXR4-4B2 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.6–3.0 µM (tested) |
| Rna Concentration | 0.6–3.0 µM (1:1 conjugate) |
| Mixing Ratio | |
| Formulation Format | covalent conjugate (disulfide) |
| Formulation Components | RXR4-4B2–PNA705 disulfide conjugate |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 (splice-correction luciferase reporter) |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction / luciferase reporter |
| Output Value | EC50 > 3 µM (RT-PCR); luciferase barely above background up to 3 µM |
| Output Units | |
| Output Notes | 8-Arg 4-branched conjugate showed very poor splice-redirecting activity in HeLa pLuc705. |
| Toxicity Notes | No significant toxicity in HeLa pLuc705 up to 3 µM (MTS viability assay). |
| Curation Notes |