Sequence: 4-branched dendrimer; total 16 Arg; multiple RXR motifs per arm; C-terminal Ahx-βAla-Cys
| Experiment Id | EXP001598 |
|---|---|
| Paper | Synthesis and Splice-Redirecting Activity of Branched, Arginine-Rich Peptide Dendrimer Conjugates of |
| Peptide | RXR8-4B1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | covalent conjugate (disulfide) |
| Formulation Components | RXR8-4B1–PNA705 disulfide conjugate |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A375SM-Luc705-B (melanoma luciferase reporter) |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction / luciferase reporter |
| Output Value | EC50 0.51 ± 0.03 µM (RT-PCR splice correction) |
| Output Units | |
| Output Notes | Assayed in A375SM-Luc705-B melanoma reporter cells; EC50 reported. |
| Toxicity Notes | In A375SM-Luc705-B melanoma cells, cell viability dropped significantly at ≥1 µM for 12- and 16-Arg conjugates (reported for RXR8, RXR8-2B, RXR8-4B1). |
| Curation Notes |