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EXP001609

Paper

Lipid nanocapsule functionalization by lipopeptides derived from human papillomavirus type-16 capsid for nucleic acid delivery into cancer cells (2013)

Peptide

CP (control peptide)

Sequence: CLVEETSFIDAGAPS

RNA

pDNA

All experiment fields

Experiment Id EXP001609
Paper Lipid nanocapsule functionalization by lipopeptides derived from human papillomavirus type-16 capsid
Peptide CP (control peptide)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence High
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 10 µg/mL (plasmid during COS-7 transfection)
Mixing Ratio DNA does not complex with CP-LNCs in gel assay; DNA incubated with LNCs for transfection comparisons
Formulation Format peptide-functionalized lipid nanocapsule + pDNA mixture (no complexation observed)
Formulation Components Lipid nanocapsules (LNCs; Labrafac® WL1349 core, Solutol® HS15, Lipoïd® S75-3) surface-functionalized via DSPE-PEG2000-peptide (post-insertion method 2)
Size Nm 58.50
Zeta Mv -28.40
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells COS-7 (luciferase assay); uptake of LNC+DNA also shown in U87MG
Animal Model
Administration Route
Output Type luciferase expression (luminescence)
Output Value No detectable luciferase expression
Output Units
Output Notes CP-LNCs did not complex DNA and did not yield luciferase expression.
Toxicity Notes
Curation Notes