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EXP001613

Paper

Lipid nanocapsule functionalization by lipopeptides derived from human papillomavirus type-16 capsid for nucleic acid delivery into cancer cells (2013)

Peptide

CP (control peptide)

Sequence: CLVEETSFIDAGAPS

RNA

siRNA

All experiment fields

Experiment Id EXP001613
Paper Lipid nanocapsule functionalization by lipopeptides derived from human papillomavirus type-16 capsid
Peptide CP (control peptide)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence High
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 25 nM (during cell treatment)
Mixing Ratio Cell treatment: 25 nM siRNA + 100 µg/mL LNCs (2 h, serum-free)
Formulation Format siRNA + CP-LNC (no complexation observed)
Formulation Components Lipid nanocapsules (LNCs; Labrafac® WL1349 core, Solutol® HS15, Lipoïd® S75-3) surface-functionalized via DSPE-PEG2000-peptide (post-insertion method 2)
Size Nm 60.50
Zeta Mv -22.90
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells U87MG (Bcl-2 protein by flow cytometry)
Animal Model
Administration Route
Output Type flow cytometry (protein knockdown)
Output Value No significant Bcl-2 reduction
Output Units
Output Notes CP-LNCs did not deliver siRNA effectively; no functional silencing observed.
Toxicity Notes
Curation Notes