Sequence: (Dip-R)5
| Experiment Id | EXP001615 |
|---|---|
| Paper | Amphiphilic Cell-Penetrating Peptides Containing Natural and Unnatural Amino Acids as Drug Delivery |
| Peptide | [DipR]5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | High |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10 |
| Rna Concentration | 0.036 |
| Mixing Ratio | N/P = 20 |
| Formulation Format | non-covalent peptide/siRNA complex (electrostatic; N/P-defined) |
| Formulation Components | [DipR]5 + fluorescent-labeled siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 (human breast cancer) |
| Animal Model | |
| Administration Route | |
| Output Type | uptake (flow cytometry; mean fluorescence intensity fold-change vs siRNA alone) |
| Output Value | 50 |
| Output Units | |
| Output Notes | After 24 h incubation, [DipR]5 increased F'-siRNA uptake ~50-fold vs siRNA alone at N/P 20 in MDA-MB-231 cells. |
| Toxicity Notes | Minimal cytotoxicity reported at 10 µM peptide for 3 h and 24 h incubations (uptake studies conducted at least-toxic dose). |
| Curation Notes |