Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate (2022)
Sequence: Fusion carrier: HIV-1 Tat CPP (core sequence YGRKKRRQRRR) fused to U1A RNA-binding protein; rose bengal (RB) conjugated (sonosensitizer). Exact full-length sequence not provided in paper.
| Experiment Id | EXP001621 |
|---|---|
| Paper | Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate |
| Peptide | TatU1A-RB |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 2 µM TatU1A-RB (cell treatment, 3 h incubation) |
| Rna Concentration | 0.2 µM shGFP (cell treatment, 3 h incubation) |
| Mixing Ratio | TatU1A-RB:shRNA = 10:1 molar (2 µM : 0.2 µM in cell treatment) |
| Formulation Format | Protein/ RNA complex via U1A RNA-binding domain (endocytosis uptake; ultrasound-triggered escape) |
| Formulation Components | TatU1A-RB + shRNA (U1A-binding sequence) in T buffer; no additional nanoparticles/excipients reported |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-EGFP (CHO cells stably expressing destabilized EGFP); CHO used for shRNA-Fam imaging |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro functional RNAi |
| Output Value | EGFP fluorescence decreased to ~50% vs non-treated cells after ultrasound (US) with TatU1A-RB + shGFP; no significant knockdown without US |
| Output Units | |
| Output Notes | US-dependent cytosolic dispersion of shRNA-Fam observed (10–15 min US). RNAi requires shGFP and US; controls (US alone, TatU1A-RB alone, shControl) did not knock down. US: 1 MHz pulsed, duty cycle 30%, ~15 min (0.3 W/cm² in imaging; 0.3–0.5 W/cm² reported for knockdown/blood-cell experiments). |
| Toxicity Notes | Not quantitatively reported in paper/SI; focus on delivery/knockdown. (No explicit cytotoxicity numbers provided.) |
| Curation Notes |