Sequence: PFVYLIRFHRFHRFHGGGSSSQLSRP
| Experiment Id | EXP001650 |
|---|---|
| Paper | Structure-guided design of nucleosome-inspired nanoparticles for overcoming pulmonary barriers in fi |
| Peptide | MNM |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | N/P 4–20 (screen); MNM NPs typically N/P=20 for mechanistic studies |
| Formulation Format | peptide/siRNA nanoparticles (MPS-modified variants) |
| Formulation Components | peptide + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | 10.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U2OS-RFP; A549; HBE135-E6E7 (toxicity for selected variants) |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | Highest gene silencing among optimized variants; avoids lysosomal colocalization; BafA1-sensitive. |
| Output Units | |
| Output Notes | Second-stage optimization with hydrophilic mucus-penetrating sequences; MNM (P29) reported as best performer. |
| Toxicity Notes | |
| Curation Notes |