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EXP001654

Paper

Structure-guided design of nucleosome-inspired nanoparticles for overcoming pulmonary barriers in fibrotic lung gene therapy (2025)

Peptide

MN

Sequence: PFVYLIRFHRFHRFH

RNA

siRNA

All experiment fields

Experiment Id EXP001654
Paper Structure-guided design of nucleosome-inspired nanoparticles for overcoming pulmonary barriers in fi
Peptide MN
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence medium
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio Repeated dosing; N/P typically 20 for MNM NPs (see paper)
Formulation Format intratracheal peptide/siRNA assemblies
Formulation Components MN + siTGF-β1
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model C57BL/6J pulmonary fibrosis model (bleomycin, incl. 1.5 U/kg and 3 U/kg fast-progressive model)
Administration Route intratracheal
Output Type in vivo therapeutic effect
Output Value Survival improvement and reduced fibrosis; gene expression suppression (qPCR/WB) reported.
Output Units
Output Notes MN–siTGF-β1 complexes showed therapeutic benefit in aggressive PF model (survival improvement).
Toxicity Notes
Curation Notes