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EXP001655

Paper

Structure-guided design of nucleosome-inspired nanoparticles for overcoming pulmonary barriers in fibrotic lung gene therapy (2025)

Peptide

MNM

Sequence: PFVYLIRFHRFHRFHGGGSSSQLSRP

RNA

siRNA

All experiment fields

Experiment Id EXP001655
Paper Structure-guided design of nucleosome-inspired nanoparticles for overcoming pulmonary barriers in fi
Peptide MNM
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration
Mixing Ratio Repeated dosing; N/P typically 20 for MNM NPs (see paper)
Formulation Format intratracheal peptide/siRNA assemblies
Formulation Components MNM + siTGF-β1
Size Nm 80.00
Zeta Mv 10.00
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model C57BL/6J pulmonary fibrosis model (bleomycin, incl. 1.5 U/kg and 3 U/kg fast-progressive model)
Administration Route intratracheal
Output Type in vivo therapeutic effect
Output Value Survival improvement and reduced fibrosis; gene expression suppression (qPCR/WB) reported.
Output Units
Output Notes MNM–siTGF-β1 NPs improved survival and reduced fibrosis/collagen; downregulated PF genes.
Toxicity Notes
Curation Notes