Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery (2009)
Sequence: N/A (non-peptidic guanidine-based molecular transporter)
| Experiment Id | EXP001705 |
|---|---|
| Paper | Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery |
| Peptide | 3 (C30, different linker) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Surface ligand STR-R8 5 mol%; transporter used as condenser (amount set by N/P=3) |
| Rna Concentration | 0.4 µg pDNA per well (24-well plate) |
| Mixing Ratio | pDNA condensed at N/P=3; STR-R8 5 mol% (surface); 0.4 µg DNA per well |
| Formulation Format | Core–shell MEND: condensed pDNA core + lipid envelope, surface-modified with STR-R8 |
| Formulation Components | Core: pDNA condensed with transporter at N/P=3; Envelope: DOPE/CHEMS (9:2 molar); Surface ligand: STR-R8 (5 mol% of total lipid) |
| Size Nm | 259.00 |
| Zeta Mv | 31.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro transfection (luciferase expression) |
| Output Value | Luciferase expression in HeLa after 24 h; 1a (C12) core produced the highest expression among transporters and exceeded PLL-core control; longer lipid-chain condensers gave lower expression. |
| Output Units | |
| Output Notes | Transfection: 3 h serum-free, then 21 h with 10% FBS; luciferase measured as RLU/mg protein. |
| Toxicity Notes | |
| Curation Notes |