Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery (2009)
STR-R8 (stearylated octaarginine)
Sequence: Stearyl-(Arg)8 (STR-R8)
| Experiment Id | EXP001709 |
|---|---|
| Paper | Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery |
| Peptide | STR-R8 (stearylated octaarginine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | STR-R8 (core + 5 mol% surface) |
| Rna Concentration | 0.4 µg siRNA per well |
| Mixing Ratio | N/P=3 for condensation; STR-R8 surface 5 mol%; 0.4 µg siRNA per well |
| Formulation Format | siRNA MEND: condensed siRNA core + lipid envelope, surface-modified with STR-R8 |
| Formulation Components | Core: siRNA condensed with STR-R8 at N/P=3; Envelope: DOPE/PA (7:2); Surface STR-R8 (5 mol%) |
| Size Nm | 250.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa-luciferase stable cells |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro gene knockdown (luciferase silencing) |
| Output Value | Strong luciferase knockdown (used as comparator); transporter 1a-core MEND exceeded this STR-R8-core system. |
| Output Units | |
| Output Notes | Reference system from prior work; included as comparator in Fig. 5. |
| Toxicity Notes | |
| Curation Notes |