Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery (2009)
Sequence: N/A (non-peptidic guanidine-based molecular transporter)
| Experiment Id | EXP001713 |
|---|---|
| Paper | Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery |
| Peptide | 2 (surface ligand) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Ligand 8 mol% of lipids |
| Rna Concentration | 0.4 µg pDNA per well |
| Mixing Ratio | PLL condense N/P=2.4; ligand 8 mol% of lipids; 0.4 µg DNA per well |
| Formulation Format | MEND with PLL/pDNA core and surface-modifying ligand |
| Formulation Components | Core: pDNA condensed with PLL (N/P=2.4); Envelope: DOPE/CHEMS (9:2); Surface ligand: transporter or STR-R8 (8 mol% of total lipid) |
| Size Nm | 404.00 |
| Zeta Mv | 33.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; NIH3T3 (comparison for optimized ligand) |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro transfection (luciferase expression) |
| Output Value | Surface ligands with longer lipid chains improved transfection vs STR-R8; compound 3 was the best surface ligand (≈4× vs STR-R8 in HeLa) and outperformed Lipofectin in HeLa and NIH3T3. |
| Output Units | |
| Output Notes | Transfection measured at 24 h. Ligand role differs from condenser role: 1a best as condenser; 3 best as surface ligand. |
| Toxicity Notes | |
| Curation Notes |