Sequence: Not specified as a single sequence (composition-based guanidinated α-helical polypeptide; DP≈113; 15 mol% benzyl aromatic).
| Experiment Id | EXP001724 |
|---|---|
| Paper | Self-Assisted Membrane-Penetrating Helical Polypeptides Mediate Anti-Inflammatory RNAi against Myoca |
| Peptide | P-Ben |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | Polypeptide/siRNA weight ratio = 20 used for intracardial efficacy injections (P-Ben/siRAGE); imaging/uptake comparisons used w/w=15 for Cy3-siRNA. |
| Formulation Format | Helical polypeptide/siRNA polyplexes by simple mixing and incubation |
| Formulation Components | Intramyocardial injection polyplex: P-Ben/siRAGE (w/w=20) at 150 µg siRNA/kg; prepared by mixing and 30 min incubation. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Male Sprague–Dawley rats (myocardial ischemia–reperfusion injury via LAD ligation) |
| Administration Route | Intramyocardial injection (below LAD site) 10 min after reperfusion |
| Output Type | in vivo functional knockdown + phenotypic benefit |
| Output Value | ~84–85% RAGE mRNA knockdown at 24 h; reduced TNF-α and IL-6; reduced infarct size (~6.9%), fibrosis (~11.8%), apoptosis (~12.9%); improved EF/FS near normal. |
| Output Units | |
| Output Notes | Rat myocardial IR model (LAD ligation 40 min + reperfusion). Intramyocardial injection 10 min post reperfusion: polyplexes at 150 µg siRNA/kg (50 µL/rat). |
| Toxicity Notes | Histology indicates reduced damage vs controls; no acute toxicity signals reported for P-Ben at used dose. |
| Curation Notes |