Sequence: Not specified as a single sequence (composition-based guanidinated α-helical polypeptide; DP≈113; no aromatic).
| Experiment Id | EXP001725 |
|---|---|
| Paper | Self-Assisted Membrane-Penetrating Helical Polypeptides Mediate Anti-Inflammatory RNAi against Myoca |
| Peptide | P-Homo |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | P-Homo/siRAGE w/w=20 for intracardial injections (150 µg siRNA/kg); imaging used w/w=15 |
| Formulation Format | Helical polypeptide/siRNA polyplexes by simple mixing and incubation |
| Formulation Components | Intramyocardial injection polyplex: P-Homo/siRAGE (w/w=20) at 150 µg siRNA/kg. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Male Sprague–Dawley rats (myocardial IR injury, LAD ligation) |
| Administration Route | Intramyocardial injection 10 min after reperfusion |
| Output Type | in vivo gene knockdown (less effective than P-Ben) |
| Output Value | ~55% RAGE mRNA knockdown at 24 h; higher infarct size (~35.7%) and fibrosis/apoptosis vs P-Ben group. |
| Output Units | |
| Output Notes | Rat myocardial IR model (LAD ligation 40 min + reperfusion). Intramyocardial injection 10 min post reperfusion: polyplexes at 150 µg siRNA/kg (50 µL/rat). |
| Toxicity Notes | |
| Curation Notes |