Characterization and evaluation of a peptide-based siRNA delivery system in vitro (2017)
Sequence: Ac-RLWHLLWRLWRRLHRLLR-NH2
| Experiment Id | EXP001737 |
|---|---|
| Paper | Characterization and evaluation of a peptide-based siRNA delivery system in vitro |
| Peptide | C6M3 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Complexes prepared at peptide/siRNA molar ratios (MR) 10:1–40:1; typical uptake imaging MR20; gene silencing MR40. |
| Rna Concentration | 100 nM siRNA for uptake and gene silencing assays |
| Mixing Ratio | Peptide:siRNA molar ratio 10:1–40:1 (MR40 used for best knockdown) |
| Formulation Format | Noncovalent peptide/siRNA complexes (polyplex nanoparticles) |
| Formulation Components | C6M3 peptide + siRNA, incubated ~20 min (Opti-MEM for cell work; RNase-free water for physchem) |
| Size Nm | 72.00 |
| Zeta Mv | 30.50 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-K1 (Chinese hamster ovary) |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro uptake + gene knockdown |
| Output Value | Uptake increased with MR; at MR40, higher % Cy3+ cells than Lipofectamine 2000. GAPDH mRNA knockdown ~69% (qRT-PCR) at MR40 (100 nM siRNA). |
| Output Units | |
| Output Notes | Cells treated 4 h in Opti-MEM with complexes, then 48 h in complete medium. Uptake assessed by fluorescence microscopy (Cy3-siRNA localized perinuclear) and flow cytometry after heparin wash. |
| Toxicity Notes | Low cytotoxicity: >90% CHO-K1 viability for C6M3 ± siRNA across MR10–MR40; Lipofectamine 2000 ~57% viability. |
| Curation Notes |