PCL-R15 (PCL3000-b-R15 amphiphilic block polymer)
Sequence: Not a single peptide sequence; PCL3000-b-R15 block copolymer.
| Experiment Id | EXP001739 |
|---|---|
| Paper | Polyarginine-Mediated siRNA Delivery: A Mechanistic Study of Intracellular Trafficking of PCL-R15/si |
| Peptide | PCL-R15 (PCL3000-b-R15 amphiphilic block polymer) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | no |
| Peptide Concentration | |
| Rna Concentration | 100 nM |
| Mixing Ratio | N/P = 40 |
| Formulation Format | PCL-R15/siRNA nanoplexes (micelle-based) |
| Formulation Components | Thin-film hydration to form PCL-R15 micelles; mix with siRNA in 5% glucose; vortex; stand 10 min. |
| Size Nm | 80.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa (human cervical cancer) |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro negative control (no silencing) |
| Output Value | No VEGF downregulation vs untreated controls (sequence specificity control). |
| Output Units | |
| Output Notes | Same transfection workflow as siVEGF condition. |
| Toxicity Notes | As above for PCL-R15/siRNA at 100 nM. |
| Curation Notes |