COPA and SLC4A4 are Required for Cellular Entry of Arginine-Rich Peptides (2014)
9R (poly-arginine, nona-arginine)
Sequence: RRRRRRRRR (9R; FITC-conjugated)
| Experiment Id | EXP001741 |
|---|---|
| Paper | COPA and SLC4A4 are Required for Cellular Entry of Arginine-Rich Peptides |
| Peptide | 9R (poly-arginine, nona-arginine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10 µM FITC-peptide for 1 h incubation (uptake assay/screening). |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | Free fluorescent peptide uptake assay (no RNA payload). |
| Formulation Components | FITC-conjugated CPP incubated with cells; uptake quantified by confocal microscopy + image analysis (MetaMorph). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa (primary screen); additional validation in HepG2, U-87 MG, HEK293 (supplement). |
| Animal Model | |
| Administration Route | |
| Output Type | cellular uptake/internalization (fluorescence microscopy) |
| Output Value | Knockdown of COPA or SLC4A4 significantly reduced intracellular fluorescence signal vs scrambled siRNA; double knockdown further reduced uptake at lower siRNA doses. |
| Output Units | |
| Output Notes | Genome-wide siRNA screen of membrane-associated genes; cells transfected 72 h then treated with FITC-9R for 1 h at 37°C; uptake quantified per-cell fluorescence intensity. |
| Toxicity Notes | |
| Curation Notes |