FA-R9-FPcas-3 (FMN-17 carrier peptide)
Sequence: FA-(Arg)9-(click-linker)-Ac-DEVD-PABA-mCy (NIR dye)
| Experiment Id | EXP001743 |
|---|---|
| Paper | Renal-targeting peptide-microRNA nanocomplex for near IR imaging and therapy of renal ischemia/reper |
| Peptide | FA-R9-FPcas-3 (FMN-17 carrier peptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Typically 4 mM peptide carrier in formulations (as reported for imaging/uptake conditions); flow cytometry used 20 µM FMN-17 for 24 h in apoptosis assay |
| Rna Concentration | 100 nM miRNA mimic (typical in vitro transfection); |
| Mixing Ratio | Peptide:miRNA molar ratios tested 10:1–40:1; commonly 20:1 for FMN-17 |
| Formulation Format | Self-assembled peptide/miRNA nanocomplex (FMN) |
| Formulation Components | FA-R9-FPcas-3 peptide + miR-17-5p mimic (self-assembled FMN-17) |
| Size Nm | 200.00 |
| Zeta Mv | 5.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HK-2 human renal tubular epithelial cells (H/R model); selectivity assessed vs FHS74INT (intestinal) and L-O2 (hepatic) cells |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro functional effect (anti-apoptosis + caspase-3 activity imaging) |
| Output Value | In HK-2 hypoxia/reoxygenation model, FMN-17 increased intracellular miR-17-5p and reduced apoptosis (Annexin V/PI, TUNEL), downregulated cleaved caspase-3, reduced ROS and urinary NAG; lower NIR turn-on signal consistent with reduced caspase-3 activation. |
| Output Units | |
| Output Notes | Best delivery at peptide:miRNA molar ratios around 20:1 (qPCR). Confocal shows intracellular Cy3-miRNA signal with LysoTracker staining (uptake). Nanocomplex size ~200–300 nm with zeta ~−5 to +8 mV across ratios; TEM shown for one ratio. |
| Toxicity Notes | No strong cytotoxicity reported for FMN-17 at working concentrations; compared against Lipofectamine controls in miRNA delivery. |
| Curation Notes |